Cineteca Matadero, located at Plaza de Legazpi, 8, is a movie theater founded in 2011 in a space that once held the old refrigeration units and boiler of the municipal slaughterhouse. It has three salons, with one seating 224 people, and another 129 and the third seating 65 people. Cineteca Matadero hosted a queer film series as part of extended 2017 World Pride events. The memories transfeministas queer / cuir cycle took place in mid-September. Among the films that appeared at the event was The Watermelon Woman, a film based on the story of black lesbian Cheryl who struggles to make a documentary about black 1930s film actress Fae Richards, commonly referred to as the watermelon woman. The eighth edition of the Nuestra de Cine Lésbico was held at Cineteca Matadero from 23 to 26 May 2019. Among the films screened were Tell It To The Bees, Carmen y Lola, Rafiki, Cloudburst, Las Hijas del Fuego, Lesbofobia, Cárceles Bolleras, Small Talk and La Vida de Adele. The film festival was organized by Fundación Triángulo.

Villaverde Entiende y Usera con Orgullo both held Pride marches on 2 July 2016 with the goal of showing that gay rights and LGBT visibility should not and do not die on the periphery of Madrid. Their march started in the south and ended at Atocha, joining the bigger Orgullo events in the city. They were supported by neighborhood organizations including La Unidad de Villaverde Este, La Unidad de San Cristóbal, AVIB, AV La Incolora, AV Los Rosales, AV Zofío y AV Orcasitas, and the Delegación de la FAPA de Villaverde. Their march started at the Metro Villaverde Bajo-Cruce, continued to Metro San Fermín-Orcasur in Usera, and onwards to Metro Legazpi.